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Millipore
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Journal: Investigative Ophthalmology & Visual Science
Article Title: Distinct Transcriptomic Profiles of Cultured Anterior and Posterior Populations of Human Infant Scleral Fibroblasts: Including Dopamine Receptors
doi: 10.1167/iovs.66.5.29
Figure Lengend Snippet: D1-like receptor activity promotes the inhibitory effect of DA on cell contraction, whereas D2-like receptor activity, particularly that of DRD2 in the posterior sclera, impedes it. ( a – p ) The cell contraction rates at 24 hours for anterior ( a – h ) and posterior ( i – p ) scleral fibroblasts from HSF12 and HSF13 were measured following treatment with a range of DA receptor antagonists and agonists, administered 1 hour prior to the addition of DA. One experiment was conducted with three replicate wells for each compound tested, using two biological samples (HSF12 and HSF13; total n = 6). Results from the two cell lines were averaged and presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-way ANOVA).
Article Snippet: The antibodies used were as follows:
Techniques: Activity Assay
Journal: Nutrition Research and Practice
Article Title: Dopamine and serotonin alterations by Hizikia fusiformis extracts under in vitro cortical primary neuronal cell cultures
doi: 10.4162/nrp.2023.17.3.408
Figure Lengend Snippet: HF, Hizikia fusiformis extract treatment; VEH, vehicle treatment (control); DRD1-5, dopamine D1–5 receptors; DAT, dopamine transporter; DAPI, 4′,6-diamidino-2-phenylindole; NF-L, neurofilament light chain; SERT, serotonin transporter; TH, tyrosine hydroxylase; TPH, tryptophan hydroxylase; SYP, synaptophysin; 5HT1A and 1B, serotonin 1A and 1B receptors; MAP2, microtubule-associated protein 2.
Article Snippet: In this study, we used primary antibodies for DA transmission: mouse monoclonal anti-tyrosine hydroxylase (TH; Abcam; catalog #ab129991), rabbit polyclonal anti-DAT (Santa Cruz; catalog #sc-14002),
Techniques:
Journal: Nutrition Research and Practice
Article Title: Dopamine and serotonin alterations by Hizikia fusiformis extracts under in vitro cortical primary neuronal cell cultures
doi: 10.4162/nrp.2023.17.3.408
Figure Lengend Snippet: DRD1–5, dopamine D1–5 receptors; HF, Hizikia fusiformis extract treatment; VEH, vehicle treatment (control); DAT, dopamine transporter; TH, tyrosine hydroxylase.
Article Snippet: In this study, we used primary antibodies for DA transmission: mouse monoclonal anti-tyrosine hydroxylase (TH; Abcam; catalog #ab129991), rabbit polyclonal anti-DAT (Santa Cruz; catalog #sc-14002),
Techniques:
Journal: Nutrition Research and Practice
Article Title: Dopamine and serotonin alterations by Hizikia fusiformis extracts under in vitro cortical primary neuronal cell cultures
doi: 10.4162/nrp.2023.17.3.408
Figure Lengend Snippet: A summary of BF 10 with post-hoc pair wise comparisons
Article Snippet: In this study, we used primary antibodies for DA transmission: mouse monoclonal anti-tyrosine hydroxylase (TH; Abcam; catalog #ab129991), rabbit polyclonal anti-DAT (Santa Cruz; catalog #sc-14002),
Techniques: